School Improvement in Maryland
Regulation of an Enzyme
. 5 E's Lesson Plan.
Task Overview.
Teacher Resources.

5 E's Lesson Plan

I. Engagement: The activities in this section capture the students’ attention, stimulate their thinking, and help them access prior knowledge.

Enzyme Specificity Class Activity
and/or Pineapple/Jell-O™ Lab

1.2.1, 1.2.2, 3.1.1, 3.1.2
II. Exploration: In this section, students are given time to think, plan, investigate, and organize collected information. Beano™ and Lactaid™ Action Lab, by Vernier or Teaching Enzyme Action Using CBL Technology or Proof of Enzyme Action Lab 1.2.3, 1.2.5, 1.3.1, 1.3.2, 1.3.3, 1.4 (exc. ind. 5), 1.5.1-1.5.4, 1.6.1, 1.6.4, 1.7.1, 3.1.1, 3.1.2
III. Explanation: Students are now involved in an analysis of their exploration. Their understanding is clarified and modified because of reflective activities. Students will be guided through a discussion of enzyme action (enzymes, substrates, co enzymes) based upon Section II activities. 1.2.5, 3.1.1, 3.1.2
IV. Extension: This section gives students the opportunity to expand and solidify their understanding of the concept and/or apply it to a real world situation. Enzyme Action: Testing Catalase Activity (Vernier), Observing the Effect of Concentration on Enzyme Activity and the Effect of Temperature on Enzyme Activity 1.2.3, 1.2.5, 1.3.1, 1.3.2, 1.3.3, 1.4 (exc. ind. 5), 1.5.1-1.5.4, 1.6.1, 1.6.4, 1.7.1, 3.1.1, 3.1.2
V. Evaluation: This performance- based helps the students to connect all of the pieces of information involved in these lessons. Enzyme Graph Application: Provides students with a data set comparing time to enzyme concentration. Students will create and analyze their graph, and predict the effect of a change in enzyme concentration, pH or temperature on enzyme activity. 1.1.2, 1.4.2, 1.4.4, 1.5.1, 1.5.2, 3.1.2, 3.2.2

  • Addresses concept of Enzymes/Substrates/Regulation
  • Possible assessment items can be found at the end of this packet.

Task Overview

This is a unit which covers the effects of various factors on the activity of enzymes. It is also a means of reinforcing the process skills involved in the scientific method.

To introduce the topic and unit, two activities can be used: “Enzyme Specificity” or “Pineapple/Jell-OTM Lab”. There are three choices for the Exploration: “Beano™ and Lactaid™ Action”, “Teaching Enzyme Action Using CBL Technology” and “Proof of Enzyme Action”. It is recommended that students perform at least two of these activities, one of which should be the CBL laboratory to introduce students to the use of current technology to gather data. The complete activity is not included here, however the teacher can easily locate the source document (click here for links to source document). An Extension activity can also be obtained at the same site. Assessment items are included in the Evaluation section that mirror those that are found in the State High School Assessment.

The unit covers the following concepts and topics:
  • The correlation between the structure and function of biologically important molecules and their relationship to cell processes, specifically, factors that affect enzyme activity:
    • temperature
    • pH
    • concentration of enzyme
  • Posing scientific questions and suggesting experimental procedures
  • Carrying out investigations and employing instruments and materials
  • Analyzing data
  • Communicating information
  • Using mathematical processes
  • Connecting biology to mathematics and real-world applications


   Suggested Materials:
  • 5% Sugar solutions
  • Amylase solution
  • Beakers
  • Benedict’s solution
  • Blender
  • Calf’s liver - fresh (OR substitute raw white potato)
  • Card stock
  • CBL/TI System
  • Cheesecloth
  • Distilled water
  • Envelopes
  • Erlenmeyer flasks - 125 mL (OR substitute 250 mL)
  • Eye droppers (pipettes)
  • Food cards
  • Gelatin cubes
  • Glucose test paper
  • Graduated cylinders
  • Hot plate
  • Hydrogen peroxide solution (3%)
  • Locks and keys (15 assorted)
  • pH buffers (pH 4, 7, and 10)
  • Pestle (or device for macerating liver)
  • Pineapple juice (canned)
  • Pineapple juice (fresh or frozen)
  • Potassium iodide solution (I2KI)
  • Shallow dish or pan
  • Starch solution
  • Vernier Gas Pressure Sensor
  • Vernier Temperature Probe or Thermometer
  • Yarn
  • Yeast suspension
* Refer to “Beano™ and Lactaid™” Lab for complete list of required materials.
* Refer to “Enzyme Action: Testing Catalase Activity” Lab for complete list of required materials.
* Refer to “Proof of Enzyme Action” Lab for for complete list of required materials.
* Refer to “Teaching Enzyme Action Using CBL Technology” Lab for complete list of required materials.
*NOTE: This list of materials is NOT complete. Teachers should refer to the actual lab references for a complete list of required materials.

Teacher Resources

1a. “Enzyme Specificity: Introduction to Enzymes”
1b. “Pineapple/Jell-O™ Lab”
2a. “Beano™ and Lactaid™ Action”
2b. (Alternative)
“Teaching Enzyme Action Using CBL Technology”

2c. (Alternative)
“Proof of Enzyme Action”
4. “Enzyme Action: Testing Catalase Activity”
5. “Factors That Affect the Activity of Enzymes”

Assessment Items
Printable version (Acrobat 226k)
Michelle Thornton
High School Biology Teacher
Linganore High School
Frederick County, Maryland